Rice 1Cys‐peroxiredoxin over‐expressed in transgenic tobacco does not maintain dormancy but enhances antioxidant activity

[摘要]

Possible functions that have been proposed for the plant 1Cys-peroxiredoxin, include activity as a dormancy regulator and as an antioxidant. The transcript level of ice ys-eroxiedoin (R1C-Prx) rapidly decreased after imbibition of rice seeds, but the protein was detected for 15 days after imbibition. To investigate the function of this protein, we generated transgenic tobacco plants constitutively expressing the R1C-Prx gene. The transgenic R1C-Prx plants showed a germination frequency similar to control plants. However, the transgenic lines exhibited higher resistance against oxidative stress, suggesting that antioxidant activity may be its primary function.

[学科分类] 生物化学/生物物理

[关键词] Antioxidant activity;Rice 1Cys-peroxiredoxin;Seed dormancy;Transgenic rice 1Cys-peroxiredoxin tobacco;ABA;abscisic acid;CaMV;cauliflower mosaic virus;DNP-hydrazone;2;4-dinitrophenylhydrazone;MS;Murashige and Skoog;Prx;peroxiredoxin;R1C-Prx;rice 1Cys-peroxiredoxin;ROS;reactive oxygen species