The first peak of the UVB irradiation‐dependent biphasic induction of vascular endothelial growth factor (VEGF) is due to phosphorylation of the epidermal growth factor receptor and independent of autocrine transforming growth factor α

[摘要]

Ultraviolet B (UVB) irradiation, the major damaging component of sunlight, has earlier been reported to enhance cutaneous angiogenesis in chronically sun-exposed skin. We herein provide first evidence for a biphasic induction of the vascular endothelial growth factor (VEGF) following UVB irradiation of the human epidermal cell line HaCaT. The first VEGF peak occurred on mRNA level at 1 h and on protein level at 4 h postirradiation and is fully mediated by the UVB-dependent phosphorylation of the epidermal growth factor receptor, which subsequent to its phosphorylation also initiates at least in part the synthesis of transforming growth factor α that confers as shown previously the second late VEGF peak at 8 h on mRNA and at 24 h on protein level.

[学科分类] 生物化学/生物物理

[关键词] Ultraviolet B;Vascular endothelial growth factor;Epidermal growth factor receptor phosphorylation;PD 153035;Transforming growth factor α;HaCaT cell;FGF;fibroblast growth factor;DMEM;Dulbecco's modified Eagle's medium;EGF-R;epidermal growth factor receptor;ELISA;enzyme-linked immunosorbent assay;FCS;fetal calf serum;HRP;horseradish peroxidase;KGF;keratinocyte growth factor;PBS;phosphate-buffered saline;TBS;Tris-buffered saline;TGFα;transforming growth factor α;UV;ultraviolet;UVB;ultraviolet B;VEGF;vascular endothelial growth factor