Proadrenomedullin N‐terminal 20 peptide (PAMP) elevates blood glucose levels via bombesin receptor in mice

[摘要]

We found a potent hyperglycemic effect of proadrenomedullin N-terminal 20 peptide (PAMP) after intra-third cerebroventricular administration at a dose of 10 nmol in fasted mice. PAMP has four homologous residues with bombesin (BN), a hyperglycemic peptide. PAMP showed affinity for gastrin-releasing peptide preferring receptor (GRP-R) and neuromedin B preferring receptor. The PAMP-induced hyperglycemic effect was inhibited by [D-Phe⁶, Leu-NHEt¹³, des-Met¹⁴]-BN (6–14), GRP-R specific antagonist, indicating that the hyperglycemic effect is mediated at least in part via GRP-R. Furthermore, pretreatment of α-adrenergic blocker inhibited the PAMP-induced hyperglycemia and hyperglucagonemia, suggesting that the increase of glucagon secretion through α-adrenergic activation is involved in this hyperglycemic effect of PAMP.

[学科分类] 生物化学/生物物理

[关键词] Proadrenomedullin N-terminal 20 peptide;Hyperglycemia;Bombesin;Gastrin-releasing peptide preferring receptor;α-Adrenergic activation;PAMP;proadrenomedullin N-terminal 20 peptide;BN;bombesin;GRP-R;gastrin-releasing peptide preferring receptor;i.c.v.;intra-third cerebroventricular