Structures of fucose‐containing ceramide pentasaccharides from the plasma of blood group O Le(a−b−) nonsecretors

[摘要]

A minor, Le^c blood-group inactive ceramide pentasaccharide double band fraction has been isolated from the plasma of blood group O Le(a⁻b⁻) nonsecretors. The two purified glycolipids were analysed by NMR spectroscopy, mass spectrometry and combined gas chromatography-mass spectrometry. The following structures could be established: GlcNAc(β1 → 3)Gal(β1 → 4)Glc(β1 → 1)Cer (I); Gal(β1 → 4) [Fuc(α1 → 3)]GlcNAc(β1 → 3)Gal(β1 → 4)Glc(β1 → 1)Cer (II). It must be concluded that at least part of the secretor gene-independent plasmatic H type 2 blood-group activity can be attributed to glyco-sphingolipid I, whereas substance II, originally detected in cancerous tissue, also occurs in the plasma of healthy individuals.

[学科分类] 生物化学/生物物理

[关键词] Blood group;Glycosphingolipid;Human plasma;Lewis negative (non) secretor;Abbreviations and trivial names;are essentially the same as in [3] : Gal(β1 → 4)[Fuc(α1 → 3)] GlcNAc(β1 → 3)Gal(β1 → 4)Glc;lacto-N-fucopentaose III [19];Fuc(α1 → 2)[Gal(β1 → 4)]GlcNAc(β1 → 3)Gal(β1 → 4)Glc;lacto-N-fucopentaose IV;amu;atomic mass units