Phalloidin depletes the mitochondrial Ca2+ compartment of hepatocytes

[摘要]

In 3 h of incubation, primary cultures of rat hepatocytes attach to the substratum and exchange about 2.2 nmol ⁴⁵Ca²⁺ per mg protein. In the presence of 1 μM phalloidin, the exchanged amount of ⁴⁵Ca²⁺ was found to be decreased by about 30%. Using the uncoupling agent FCCP and the ionophore A23187 for further characterisation we determined that the ⁴⁵Ca²⁺ deficit caused by phalloidin occurs in the FCCP-sensitive compartment, i.e., the mitochondria.

[学科分类] 生物化学/生物物理

[关键词] Phalloidin;Mushroom toxin;Hepatocyte;Ca2+ pool;Mitochondria;FCS;fetal calf serum;PBS;phosphate-buffered saline;DMPHN;demethylphalloin;FCCP;carbonyl cyanide p-trifluoromethoxyphenylhydrazone