Novel crystal forms of a proteolytic core of the single‐stranded DNA‐binding protein (SSB) from E. coli

[摘要]

A proteolytic core of the Escherichia coli single-stranded DNA-binding protein (SSB) has been crystallized from phosphate buffer. Crystals suitable for X-ray data collection display monoclinic space group C2 with α = 106.8, b = 62.3 c = 100.2 Å, β = 112° and contain one tetramer of proteolysis product SSB*-A per asymmetric unit. Two other crystal forms have been obtained in the presence of the inhibitor diisopropylfluorophosphate.

[学科分类] 生物化学/生物物理

[关键词] Single-strand binding protein;Proteolysis product;Protein crystal;X-ray diffraction;SSB;single-stranded DNA-binding protein from E. coli;dsDNA;double-stranded DNA;ssDNA;single-stranded DNA;DFP;diisopropylfluorophosphate;PMSF;phenylmethylsulfonylfluoride;HPLC;high performance liquid chromatography