Specific lysis of noradrenergic synaptosomes by an antiserum to dopamine‐β‐hydroxylase

[摘要]

An antiserum to dopamine-β-hydroxylase purified from bovine adrenal medulla, acting in the presence of complement, caused the release of 12% of lactate dehydrogenase, 20% of tyrosine hydroxylase activity, and 40% of noradrenaline (NA) content from synaptosomes prepared from rat brain cerebral cortex. Uptake of [³H]NA was reduced by 54%. Anti-serum alone or complement alone were without action. The antiserum plus complement had no effect on choline uptake and did not release choline acetyltransferase, glutamate decarboxylase, dopamine or 5-hydroxytryptamine. These results suggest selective lysis of noradrenergic terminals had occurred. An enhancement of lysis was not observed when synaptosomes were stimulated with 75 K⁺ and exposed to a sub-maximal dose of antiserum, plus complement.

[学科分类] 生物化学/生物物理

[关键词] Synaptosome Noradrenergic nerve terminal Lysis Antiserum Dopamine-β-hydroxylase