Effect of 2,4‐dinitrofluorobenzene on the enzymatic properties of the b‐c 1 complex isolated from beef heart mitochondria

[摘要]

A study is presented on the effect of 2,4-dinitrofluorobenzene (DFNB) on the enzymatic properties of mitochondrial b-c ₁ complex. The chemical modification by DNFB strongly inhibits the reductase activity of the complex, this being accompanied by labelling by [³H]DNFB of core protein I, the apoprotein of b cytochromes and the 12 kDa subunit. Chemical modification by DNFB appears to alter, in particular, the domain of heme b-562.

[学科分类] 生物化学/生物物理

[关键词] Cytochrome b-C1 complex Chemical modification Amino acid residue Antimycin binding;DNFB;2;4-dinitrofluorobenzene;SDSPAGE;SDS-polyacrylamide gel electrophoresis;DQH2;durohydroquinone