Effects of GUG and AUG initiation codons on the expression of lacZ in Escherichia coli
[摘要] We have replaced the ribosomal binding site (RBS) of the lacZ gene of E. coli by those of the maturation (A) gene of phage MS2 and that of the tufA gene. Both RBSs contain a GUG initiation codon. The expression with the tufA RBS is at least 25-fold higher than with the phage RBS. Changing the GUG into AUG results in a 3-fold increase in expression in both cases. In general, higher expression is accompanied by an increase of lac-specific mRNA. It is argued that this is a consequence of the more efficient translation of the mRNA.
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[效力级别] [学科分类] 生物化学/生物物理
[关键词] Site-directed mutagenesis tufA gene Phage MS2 Maturation protein Initiation codon Translation efficiency Ribosomal binding site;Ap;ampicillin;bp;basepair(s);dNTPs;deoxyribose nucleoside 5'-triphosphates;ds;doublestranded;Km;kanamycin;nt;nucleotide(s);R;resistance;rATP;ribose adenosine 5 ' -triphosphate;RBS;ribosomal binding site;rf;replicative form;ss;singlestranded;1 × SSC;0.15 M NaCl;0.015 M Na citrate;pH 7.2 [时效性]
