A telomere binding protein in the macronuclei of the hypotrichous ciliate Stylonychia lemnae

[摘要]

Macronuclear proteins from the hypotrichous ciliate Stylonychia lemnae were isolated, separated on SDS-PAGE and electroblotted onto nitrocellulose filters. Filter binding assays with ³²P-labeled macronuclear DNA and the synthetic telomere sequence (C₄A₄/G₄T₄) in combination with competition experiments revealed strong preference of a protein with a molecular mass of approx. 40 kDa to the terminal sequence of macronuclear gene-sized molecules.

[学科分类] 生物化学/生物物理

[关键词] Western blotting;DNA-binding protein;Telomere