Rapid induction of nucleoside‐diphosphate kinase in HeLa S3 cells by human‐type interferons

[摘要]

Nucleoside-diphosphate (NDP)-kinase can be considered to be induced by human-type interferons (HuIFNs) rapidly, since an enzyme increase was detected within 2 h of incubation of HeLa S3 cells with HuIFNs, while incubation with heterologous mouse IFNs had no such effect. The enzyme increase induced by HuIFNs reached a plateau at 6 h after treatment. Actinomycin D (0.5 ) significantly blocked the enzyme increase induced by HuIFNs in the cells. A possible biological role of the enzyme in the IFN-induced biochemical events is discussed.

[学科分类] 生物化学/生物物理

[关键词] Human type interferon;Recombinant interferon;Nucleoside-diphosphate kinase;Enzyme induction (HeLa S3 cell);HulFN;human-type interferon;NDP-kinase;nucleoside-diphosphate kinase;SDS-PAGE;SDS-polyacrylamide gel electrophoresis;DTT;dithio-threitol;PMSF;phenylmethylsulfonyl fluoride