Phosphorylation of 3‐O‐methyl‐D‐glucose by yeast and beef hexokinase

[摘要]

Beef heart hexokinase and yeast hexokinase both catalyzed the phosphorylation of 3-O-[¹⁴C]methyl-D-glucose. The maximal velocity was 3 orders of magnitude lower and the K _m, for the glucose analogue 40–120 times higher than those observed with D-[U-¹⁴C]glucose. Hence, 3-O-methyl-D-glucose should not be considered as a truly nonmetabolized analogue of D-glucose.

[学科分类] 生物化学/生物物理

[关键词] Hexokinase 3-O-Methyl-D-glucose;Phosphorylation