A new hydrophobic anchor for the attachment of proteins to liposomal membranes

[摘要]

The model enzyme α-chymotrypsin covalently modified by a phosphatidylethanolamine derivative has been attached to liposomal membranes in high yield. A maximal protein/lipid ratio of 5.4 × 10⁻³ mol lipid was achieved.

[学科分类] 生物化学/生物物理

[关键词] Liposome Phosphatidylethanolamine Immobilization α-Chymotrypsin Liposome targeting;PE-phosphatidylethanolamine;PC;phosphatidylcholine;DPPC;dipalmitoylphosphatidylcholine;α-CT;α-chymotrypsin;DMSO;dimethyl sulfoxide;ATEE;N-acetyl-L-tyrosine ethyl ester