Primary structure of the β‐subunit of Torpedo californica (Na+ + K+)‐ATPase deduced from the cDNA sequence

[摘要]

DNA complementary to the Torpedo californica electroplax mRNA coding for the β-subunit of (Na⁺ + K⁺)-ATPase has been cloned by screening a cDNA library with an oligodeoxyribonucleotide probe. Nucleotide sequence analysis of the cloned cDNA has revealed that this polypeptide consists of 305 amino acid residues (including the initiating methionine). The transmembrane topology and the potential N-glycosylation sites of this polypeptide are discussed.

[学科分类] 生物化学/生物物理

[关键词] (Na+ + K+)-ATPase cDNA cloning Nucleotide sequence RNA blot hybridization analysis Transmembrane topology N-Glycosylation site Primary structure;(Na++ K+)-ATPase;sodium- and potassium-dependent adenosine triphosphatase;PTH-amino acid;phenylthiohydantoin-amino acid