Involvement of three intracellular messenger systems, protein kinase C, calcium ion and cyclic AMP, in the regulation of c‐fos gene expression in Swiss 3T3 cells

[摘要]

In quiescent cultures of Swiss 3T3 cells, platelet-derived growth factor or fibroblast growth factor known to induce both protein kinase C activation and Ca²⁺ mobilization raised c-fos mRNA. This action of the growth factors was mimicked by the specific activators for protein kinase C, such as phorbol esters and a membrane-permeable synthetic diacylglycerol, and also by the Ca²⁺ ionophores, such as A23187 and ionomycin. Prostaglandin E₁ known to elevate cyclic AMP also raised c-fos mRNA, and this action was mimicked by 8-bromo-cyclic AMP, dibutyryl cyclic AMP and forskolin. These results suggest that expression of the c-fos gene is regulated by three different intracellular messenger systems, protein kinase C, Ca²⁺ and cyclic AMP, in Swiss 3T3 cells.

[学科分类] 生物化学/生物物理

[关键词] Oncogene Growth substance Protein kinase C Ca2+ cAMP (Fibroblast);PDGF;platelet-derived growth factor;FGF;fibroblast growth factor;PGE1;prostaglandin E1;protein kinase A;cyclic AMP-dependent protein kinase;[Ca2+]i;cytoplasmic free Ca2+ concentration;TPA;12-O-tetradecanoylphorbol-13-acetate;PDBu;phorbol-12;13-dibutyrate;4α-PDD;4α-phorbol-12;13-didecanoate;OAG;1-oleoyl-2-acetylglycerol;8-Br-cAMP;8-bromo-cyclic AMP;Bt2cAMP;dibutyryl cyclic AMP;DMEM;Dulbecco's modified Eagle's medium