Occurrence of lipid and phorbol ester activated protein kinase in wheat cells

[摘要]

Chromatography of the 30000 × g supernatant of wheat cell homogenates on DEAE-cellulose yielded a fraction whose protein kinase activity was specifically stimulated by phosphatidylserine plus phorbol ester or phosphatidylserine plus diolein. Such a characteristic activation pattern established this protein kinase as being a kinase C, an enzyme which plays an important role in the regulation of cell growth and differentiation in animal cells.

[学科分类] 生物化学/生物物理

[关键词] (Wheat cell) Protein kinase C;TPA;12-O-tetradecanoylphorbol-13-acetate;DO;1;2-diolein;PS;phosphatidylserine