The lysosomal gp63‐related protein in Leishmania mexicana amastigotes is a soluble metalloproteinase with an acidic pH optimum
[摘要] Leishmania mexicana amastigotes express a lysosomal protein, which is antigenically related to the promastigote surface metalloproteinase (gp63). It is shown that the purified gp63-related protein from amastigote is also an active metalloproteinase. The pH-optimum of the enzyme is acidic, similar to lysosomal cysteine proteinases, but distinct from the neutral to basic pH-optimum of the promastigote surface proteinase. This study appears to be the first report on a metalloproteinase with a lysosomal localization.
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[效力级别] [学科分类] 生物化学/生物物理
[关键词] Metalloproteinase;Cysteine proteinase;Lysosome;Leishmania mexicana;GPI;glycosylphosphatidylmositol;gp63;glycoprotein with a molecular mass of 63 kDa;hgp63;promastigote gp63 devoid of the diacylglycerol membrane anchor;DTT;dithiothreitol;ELISA;enzyme-linked immunosorbent assay;SDS-PAGE;polyacrylamide gel electrophoresis in the presence of sodium dodecylsulfate;HEPES;N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid: CRD;cross-reacting determinant [时效性]
