Primary structure and catalytic properties of extracellular ribonuclease of bacillus circulans

[摘要]

A complete amino acid sequence of extracellular Bacillus circulans RNase was established and compared with a structure of B. amyloliquefaciens RNase. Gln¹⁵, Gly⁶⁵ and Gln¹⁰⁴ in B. amyloliquefaciens RNase were found to be replaced by Leu, Ala and Lys, respectively, in B circulans RNase. Catalytic properties of B. circulans RNase were studied.

[学科分类] 生物化学/生物物理

[关键词] RNase;Bacillus circulans;Sequence;Catalytic property;RNase Bci;Ba;Bi and Bth;ribonucleases of Bacillus circulans;B. amyloliquefaciens;B. intermedius and B. thuringiensis (EC 3.1.27.1);respectively