Molecular cloning and expression of a cDNA encoding a receptor for pituitary adenylate cyclase activating polypeptide (PACAP)

[摘要]

We have cloned and sequenced a novel cDNA (RPR7) encoding a receptor for pituitary adenylate cyclase activating polypeptide (PACAP). RPR7 was identified by PCR of rat pituitary cDNA, and full-length clones were isolated from a rat olfactory bulb cDNA library. When expressed in COS cells, RPR7 was functionally coupled to increases in intracellular cyclic adenosine monophosphate (cAMP) in response to stimulation by PACAP-38, PACAP-27, vasoactive intestinal peptide (VIP) and peptide histidine isoleucine (PHI). The order of potency of these ligands was PACAP-38 ~ PACAP-27 > VIP > PHI, suggesting that the receptor corresponds to the pharmacologically characterised PACAP Type I receptor.

[学科分类] 生物化学/生物物理

[关键词] Pituitary adenylate cyclase activating polypeptide (PACAP);G protein-linked receptor;Vasoactive intestinal peptide;Rat pituitary;Rat olfactory bulb;cDNA cloning;ACTH;adrenocorticotropic hormone;cAMP;adenosine 3':5'-cyclic monophosphate;cDNA;complementary DNA;CGRP;calcitonin gene related peptide;CRF;corticotropin releasing factor;dNTP;deoxynucleotide triphosphate;DMEM;Dulbecco's modified Eagle's medium;GHRH;growth hormone releasing hormone;GLP;glucagon-like peptide I;mRNA;messenger RNA;PACAP;pituitary adenylate cyclase activating polypeptide;PCR;polymerase chain reaction;PHI;peptide histidine isoleucine;PTH;parathyroid hormone;RNase;ribonuclease;VIP;vasoactive intestinal peptide