Analysis of filamin and α‐actinin binding to actin by the stopped flow method

[摘要]

We ascertained by the stopped flow method the overall association rate constant, k ₊₁, of filamin and α-actinin to fluorescently labelled filamentous actin of ~ 1.3 × 10⁶M⁻¹ · s⁻¹ and ~ 1.0 × 10⁶M⁻¹ · s⁻¹ as well as the overall dissociation rate constant,k ₋₁ of ~ 0.6s⁻¹ and ~ 0.4s⁻¹, respectively. The overall equilibrium constant, K, for filamin and α-actinin to actin deduced from the relation K = agree well with published data.

[学科分类] 生物化学/生物物理

[关键词] Actin;Filamin;α-Actinin;Transient kinetics