Efficient enzymatic synthesis of the sialyl‐LewisX tetrasaccharide

[摘要]

Sialyl-Lewis^x (NeuAcα2→3Galβ1→4[Fucαl→3]GlcNAc] has been identified as a ligand for E-selectin, P-selectin and recently also for L-selectin. We have synthesized the sialyl-Lewis^x tetrasaccharide by total enzymatic synthesis from N-acetyllactosamine using a placental α2→3-sialyltransferase specific for type-2 chain acceptors, followed by a cloned human α1→3-fucosyltransferase (FucTV, the ‘plasma-type’ enzyme). This procedure resulted in the tetrasaccharide in a 61% overall yield.

[学科分类] 生物化学/生物物理

[关键词] Sialyl-Lex;αl→3-Fucosyltransferase;α2→3-Sialyltransferase;Selectin;α1-AGP;α1-acid glycoprotein;α3-ST;α2→3-sialyltransferase;CMP-NeuAc:Ga1β1→4G1cNAc→R;α2→3-sialyltransferase;α3-FT;α1→3-fucosyltransferase;GDP-Fuc:Ga1β1→ 4GlcNAc→R;α1→3-fucosyltransferase;sialyl-LeX;sialyl-Lewisx