Phosphorylation of elongation factor‐2 from the lepidopteran insect, spodoptera frugiperda

[摘要]

In mammalian cells, protein synthesis can be regulated at the level of elongation by the phosphorylation of elongation factor 2 (eEF-2) by a highly specific Ca²⁺/calmodulin-dependent kinase. In this report, we show that eEF-2 from a cell line derived from the insect, Spodoptera frugiperda, is a substrate for mammalian eEF-2 kinase and that phosphorylation is Ca²⁺-dependent. Furthermore, two-dimensional peptide mapping shows that the kinase phosphorylates the same sites in Spodoptera eEF-2 as those phosphorylated in the rabbit protein. However, we were unable to detect an eEF-2 kinase in Spodoptera cells.

[学科分类] 生物化学/生物物理

[关键词] Elongation factor 2;Protein synthesis;Phosphorylation;Spodoptera;(eEF-2);Eukaryotic elongation factor 2;MES;2-[N-morpholino]ethanesulphonic acid;SDS PAGE;sodium dodecyl sulphate/polyacrylamide gel electrophoresis