Purification of recombinant hepatitis delta antigen expressed in E. coli cells

[摘要]

Recombinant DNA technology enables the massive production of recombinant hepatitis delta antigen (recHDAg) retaining immunological properties and transport functions. However, purification procedures of the recombinant delta antigen have, to date, not been described in the literature. We present a purification procedure allowing one to obtain highly purified recHDAg from bacterial cells expressing the hepatitis delta antigen.

[学科分类] 生物化学/生物物理

[关键词] Hepatitis delta antigen;Fusion protein;Purification;HD;hepatitis delta;HDV;hepatitis delta virus;HBV;hepatits B virus;HDAg;delta antigen;recHDAg;recombinant delta antigen;HBsAg;hepatitis B surface antigen;TFA;trifluoroacetic acid;CH6ClN3;guanidinium chloride;CH3CN;acetonitrile;kDa;kilodalton;kb;kilobase;anti-HD;anti-HDV antibodies;BSA;bovine serum albumin;OVA;ovin serum albumin