Tissue‐specific isoforms of the bovine mitochondrial ATP synthase γ‐subunit

[摘要]

Tissue-specific isoforms of the γ-subunit of the bovine F₀F₁-ATP synthase were identified for the first time. The two isoforms, heart and liver type, were generated by alternative splicing, the liver-type RNA transcript containing a 37-nucleotide sequence as a cassette exon. Protein sequencing of the C-terminal fragments of the two isoforms of the F₁γ-subunit indicated that the liver-type isoform had an additional aspartate residue at the C terminus, not present in the heart type one.

[学科分类] 生物化学/生物物理

[关键词] F1-ATPase;ATP synthase;Isoform;Alternative splicing;RT-PCR;reverse transcription-polymerase chain reaction;F1;catalytic portion of ATP synthase;SDS-PAGE;sodium dodecyl sulfate-polyacrylamide gel electrophoresis;HPLC;high-performance liquid chromatography;DEAE;diethylaminoethyl;PTH;phenylthiohydantoin