The VIP2 receptor: Molecular characterisation of a cDNA encoding a novel receptor for vasoactive intestinal peptide

[摘要]

We have cloned and sequenced a cDNA (RPR4) encoding a new member of the secretin/calcitonin/parathyroid hormone (PTH) receptor family. RPR4 was identified by PCR of rat pituitary cDNA, and a full-length clone was isolated from a rat olfactory bulb cDNA library. When RPR4 was functionally expressed in COS 7 cells, cyclic adenosine monophosphate (cAMP) production was stimulated by vasoactive intestinal peptide (VIP), pituitary adenylate cyclase activating peptides (PACAP-38 and PACAP-27) and helodermin, with equal potency. Peptide histidine isoleucine (PHI) and rat growth hormone releasing hormone (rGHRH) also stimulated cAMP production at lower potency. This suggests that RPR4 encodes a novel VIP receptor which we have designated the VIP₂ receptor. In situ hybridisation showed that mRNA for this receptor was present mainly in the thalamus, hippocampus and in the suprachiasmatic nucleus.

[学科分类] 生物化学/生物物理

[关键词] Vasoactive intestinal peptide (VIP);G protein-linked receptor;Rat pituitary;Rat olfactory bulb;cDNA cloning;cAMP;adenosine 3′:5′-cyclic monophosphate;cDNA;complementary DNA;CGRP;calcitonin gene related peptide;CRF;corticotrophin releasing factor;dNTP;deoxynucleotide triphosphate;DMEM;Dulbecco's modified Eagle's medium;rGHRH;rat growth hormone releasing hormone;GLP;glucagon-like peptide I;mRNA;messenger RNA;PACAP;pituitary adenylate cyclase activating polypeptide;PCR;polymerase chain reaction;PHI;peptide histidine isoleucine;PTH;parathyroid hormone;RNase;ribonuclease;VIP;vasoactive intestinal peptide