Mass spectrometric evidence for a disulfide bond in aequorin regeneration

[摘要]

Tryptic digests of purified recombinant apoaequorin were analyzed, before and after reduction with DTT, by fast atom bombardment mass spectrometry. The results showed that apoaequorin contains a disulfide bond between Cys¹⁴⁵ and Cys¹⁵² and that the reduction of this bond is involved in the regeneration of aequorin.

[学科分类] 生物化学/生物物理

[关键词] Bioluminescence;Photoprotein;Ca2+-binding protein;Coelenterazine