cDNA cloning and pharmacological characterization of an opioid receptor with high affinities for κ‐subtype‐selective ligands
[摘要] The amino acid sequence of a rat opioid receptor, designated as ROR-D, has been deduced by cloning and sequencing the cDNA. The ROR-D expressed from the cDNA exhibits high affinities for ligands selective for the opioid receptor κ-subtype and low affinities for ligands selective for the δ- and μ-subtypes. RNA blot hybridization analysis indicated that ROR-D mRNA is distributed in cerebrum and brainstem but not in cerebellum.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物化学/生物物理
[关键词] Opioid receptor;cDNA cloning;cDNA expression;RNA blot hybridization analysis;Rat brain;G-proteins;guanine nucleotide-binding regulatory proteins;EKC;ethylketocyclazocine;DADLE;[d-Ala2;d-Leu5]-enkephalin;DPDPE;[d-penicillamine2;d-penicillamine2]enkephalin;DAGO;[d-Ala2;MePhe4;Gly-ol5]enkephalin [时效性]
