Identification of a new 80 k isoform of phosphatidylinositol 4‐phosphate 5‐kinase from bovine brain
[摘要] Phosphatidylinositol 4-phosphate 5-kinase is associated with bovine brain microsomes to an extent of approximately 65% of the total cellular enzyme activity. This membrane-associated kinase activity can be solubilized with Triton X-114. Following polyacrylamide gel electrophoresis in the presence of SDS the enzyme can be renaturated from gel slices in the presence of desoxycholate and Triton X-100. Catalytic activity appears at an apparent molecular weight of 80 k. Analysis of the reaction product formed by the 80 k protein reveals the existence of a 5-phosphotransferase, identifying the 80 k polypeptide as a new phosphatidylinositol 4-phosphate 5-kinase isoform.
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[效力级别] [学科分类] 生物化学/生物物理
[关键词] Phosphatidylinositol 4-phosphate 5-kinase;Bovine brain;Renaturation;PtdIns;1-(3-sn phosphatidyl)-D-myo-inositol;PtdIns4P;1-(3-sn phosphatidyl)-D-myo-inositol 4-phosphate;PtdIns(4;5)P2;1-(3-sn phosphatidyl)-D-myo-inositol 4;5-bisphosphate;GroPIns;glycerophospho-D-myo-inositol;SDS-PAGE;polyacrylamide gel electrophoresis in presence of SDS;EGF;epidermal growth factor;DOC;desoxycholate;G-protein;guanine-nucleotide-dependent regulatory protein;SDS;sodium dodecyl sulfate;PMSF;phenylmethylsulfonyl fluoride;DTE;dithioerythritol [时效性]
