Effect of okadaic acid on protein phosphorylation patterns of chicken myogenic cells with special reference to creatine kinase

[摘要]

Okadaic acid and other agents affecting cellular phosphorylation and dephosphorylation processes profoundly changed the phosphoprotein pattern of ³²P_ilabelled chicken embryonic skeletal muscle cells. The phosphorylation states of proteins in the lower molecular weight range were especially increased. Immunoprecipitation of cellular extracts with anti-creatine kinase antibodies enabled us to identify creatine kinase (CK) phosphoproteins. B-CK was phosphorylated after treating the cultures with 1-oleoyl-2-acetyl-sn-glycerol, dibutyryl-cAMP, okadaic acid and combinations thereof, but not with 1,2-dioleoyl-sn-glycerol. M-CK was also shown to be phosphorylated. The results indicated that in vivo, CK isoforms in muscle are subjected to control mediated by phosphorylation and dephosphorylation processes.

[学科分类] 生物化学/生物物理

[关键词] Phosphorylation of creatine kinase;Okadaic acid;Chicken;Myogenic cell;B- and M-CK;refer to brain- and muscle-type creatine kinase isoenzymes;respectively;CaM-kinase;Ca2+/calmodulin-de-pendent protein kinase;DAG;diacylglycerol;dbcAMP;di-butyryl-3'-5'-cAMP;OA;okadaic acid;OAG;1-oleoyl-2-acetyl-sn-glycerol;PCr;phosphocreatine;PKA;cyclic AMP-dependent protein kinase;PKC;protein kinase C;PP1 and PP2A;type 1 and type 2A phosphatase;respectively