The pH‐dependent anion‐conducting channel of the mitochondrial inner membrane is potently inhibited by zinc ions

[摘要]

Zinc is a potent reversible inhibitor of the pH-dependent anion-conducting channel in the mitochondrial inner membrane, 50% inhibition was produced by 1.5 μM added Zn²⁺ at which point free Zn²⁺ was ⩽ 10⁻⁸ M. Inhibition by Zn²⁺ is rapid but can be prevented or rapidly reversed by excess EDTA. Concentrations of Zn²⁺ higher than 4 μM caused reversal of inhibition to a variable extent depending on the anion. Under these conditions Zn²⁺ did not inhibit ribose entry, the phosphate transporter, or the pH-insensitive component of the NO₃ ⁻ uniport.

[学科分类] 生物化学/生物物理

[关键词] Anion channel;Anion uniport;Mitochondrion;Mitochondrial inner membrane;Zinc;Inhibition;IC50;concentration producing 50% inhibition;FCCP;carbonyl cyanide 4-(trifluoXXXomethoxy)phenyl-hydrazone