Calcium influx through annexin V ion channels into large unilamellar vesicles measured with fura‐2

[摘要]

A new assay for the calcium channel activity of annexin V was developed. The calcium-sensitive fluorescence indicator, fura-2, was incorporated into large unilamellar vesicles (LUV). After establishing a calcium gradient across the liposomal membranes, native or mutated annexin V was added. The resulting calcium influx into the LUV detected through the fluorescence changes of fura-2 was used as a qualitative test for the electrophysiological properties of annexin V.

[学科分类] 生物化学/生物物理

[关键词] Annexin V;Calcium channel;Liposome;Fura-2;EGTA;ethylene glycol (bis(2-aminoethyl ether)) N;N;N';N'-tetraacetic acid;EDTA;ethylene diamine tetraacetic acid;LUV;large unilamellar vesicles;MLV;multilamellar vesicles;TMA;tetramethylammonium hydroxide