Reduced content of the quinone acceptor Q Ain photosystem II complexes isolated from thylakoid membranes after prolonged photoinhibition under anaerobic conditions
[摘要] The plastoquinone content of photosystem II complexes isolated from spinach photosystem II-enriched membranes subjected to strong photoinhibitory illumination under anaerobic conditions was determined by HPLC. A pronounced decrease of the plastoquinone content was found in the photoinactivated complexes. These results corroborate earlier models in which photoinhibitory illumination is suggested to eventually lead to release of doubly reduced and protonated Q A from its site on the D2-protein.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物化学/生物物理
[关键词] Photoinhibition;Photosystem II;Plastoquinone;Chl;chlorophyll;EPR;electron paramagnetic resonance;HPLC;high pressure liquid chromatography;MES;2-(N-mor-pholino)ethanesulphonic acid;PSII;photosystem II;PQ;plastoquinone;Q Aand Q B;primary and secondary plastoquinone acceptors;respectively;P680;primary donor in photosystem II;SDS-PAGE;sodium dodecyl sulphate polyacrylamide gel electrophoresis [时效性]
