Expression of highly active sex‐inducing pheromone of Volvox carteri f. nagariensis in a mammalian cell system

[摘要]

A cDNA fragment coding for the sex-inducing glycoprotein of Volvox carteri f. nagariensis was expressed in a mammalian cell system (baby hamster kidney (BHK) cells). The transfection product exhibited a specific biological activity intermediate between the natural pheromone of the strains Volvox carteri f. nagariensis and Volvox carteri f. weismannia. Immunoblot analysis showed that the sex-inducing activity was expressed as a set of three iso-glycoproteins (35, 34 and 31 kDa).

[学科分类] 生物化学/生物物理

[关键词] Glycoprotein;Sexual inducer;Pheromone;BHK cell;Volvox carteri;BHK;baby hamster kidney;DMEM Dulbecco's Modified Eagle's Medium;FCS;fetal calf serum;G418;geneticin;PBE-94;polybuffer exchanger 94;PBS;phosphate-buffered saline.