Occurrence and sequence of a DnaJ protein in plant (Allium porrum) epidermal cells
[摘要] Antibodies raised against a purified fraction from microsomal membranes of leek epidermal cells were used to screen a λzap expression library from epidermal cells of leek plants. A near full-length clone was isolated. This cDNA contains an open reading-frame of 1,191 bp coding for a DnaJ protein (leek DNAJ 1 or LDJ1). Leek DnaJ1 represents the second protein of this type described in a pluricellular organism, the first being that sequenced from human cells.
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[效力级别] [学科分类] 生物化学/生物物理
[关键词] Plant;Allium porrum;DnaJ protein;Chaperone;Heat-shock protein;Prenylation;Zinc finger;LB;Luria-Bertani medium (Bactotryptone 10 g;Bacto yeast extract 5 g;NaCl 10 g;H2O 11;IPTG;isopropylthio-β-d-galactoside;PBS;phosphate-buffered saline;BCIP;5-bromo-4-chloro-3-indolyl phosphate;disodium salt;NBT;Nitroblue tetrazolium chloride [时效性]
