Interaction of urokinase‐type plasminogenactivator (u‐PA) with its cellular receptor (u‐PAR) induces phosphorylation on tyrosine of a 38 kDa protein
[摘要] We demonstrate by immunoprecipitation that u-PAR is associated with a 38 kDa protein that is phosphorylated on tyrosine after u-PA treatment of cells. As tyrosine phosphorylation is the hallmark of many signal transduction pathways that promote growth and differentiation, these data suggest that u-PA, besides its role as a regulatory protease, might act as a para- or autocrine hormone.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物化学/生物物理
[关键词] Plasminogen activator;Urokinase;Urokinase-type plasminogen activator receptor;Tyrosine phosphorylation;U-937 cell;Signal transduction;u-PA;urokinase-type plasminogen activator;u-PAR;u-PA receptor;PMA;phorbol 12-myristate 13-acetate;DFP;diisopropyl fluorophosphate;DSS;disuccinimidyl suberate;PBS;phosphate buffered saline;SDS;sodium dodecyl sulphate;PAGE;polyacrylamide gel electrophoresis;DTT;dithiothreitol;BSA;bovine serum albumin [时效性]
