Induction of phosphatidic acid by fibroblast growth factor in cultured baby hamster kidney fibroblasts

[摘要]

Basic fibroblast growth factor (bFGF/FGF-2) is a strong mitogenic inducer of cultured baby hamster kidney (BHK) cells. When cultured BHK cells were stimulated with FGF-2, phosphatidic acid (PA) was induced within 2 min, peaked at 5 min and gradually decreased. Phospholipase D (PLD) was also mitogenic for cultured BHK cells and this effect was mediated via PA. The possibility that PA induction by FGF-2 is an essential signaling step for BHK cell proliferation is discussed.

[学科分类] 生物化学/生物物理

[关键词] Fibroblast growth factor;Phosphatidic acid;Phospholipase D;BHK cell;BHK cells;baby hamster kidney cells;DG;diacylglycerol;EGF;epidermal growth factor;FGF-2;β-fibroblast growth factor;IPS;inositol 1;4;5-trisphosphate;PA;phosphatidic acid;PC;phosphatidylcholine;PC-PLC;phosphatidylcholine-specifie phospholipase C;PDGF;platelet-derived growth factor;PI;phosphatidylinositol;PIP;phosphatidylinositol 4-phosphate;PIP2;phosphatidylinositol 4;5-bisphosphate;PIPK;phosphatidylinositol-4-phosphate kinase;PI-PLC;phosphatidylinositol-specific phospholipase C;PI3K;phosphatidylinositol 3-kinase;PKC;protein kinase C;PLC-γ;phospholipase C-γl;PLD;phospholipase D;RasGAP;Ras GTPase activating protein