Transforming growth factors type‐β and dexamethasone attenuate group II phospholipase A2 gene expression by interleukin‐1 and forskolin in rat mesangial cells

[摘要]

Treatment of rat mesangial cells with interleukin-1β(IL-1β) and forskolin induced, in a synergistic fashion,the expression of group II phospholipase A₂ (PLA₂) mRNA, with subsequent increased synthesis and secretion of PLA₂. In contrast, interleukin-6 did not increase PLA₂ mRNA levels of PLA₂ activity. Transforming growth factor (TGF) β₁, TGFβ₂ and TGFβ₃ equipotently attenuated the IL-1β- and forskolin-induced elevation of PLA₂ mRNA, as well as PLA₂ synthesis and secretion. The glucocorticoid dexamethasone only partially suppressed the IL-1β- and forskolin-induced elevation of PLA₂ mRNA, but totally inhibited PLA₂ synthesis and secretion.

[学科分类] 生物化学/生物物理

[关键词] Phospholipase A2;Interlaukin-1;Transforming growth factor β;Dexamethasone;Phospholipase A2 mRNA;Mesangial cell;PLA2;phospholipase A2;IL- 1;interleukin-1;TNF;tumour necrosis factor;TGF;transforming growth factor