Noradrenaline release from permeabilized synaptosomes is inhibited by the light chain of tetanus toxin

[摘要]

Noradrenaline release from rat brain cortical synaptosomes permeabilized with streptolysin O can be triggered by μM concentrations of free Ca²⁺. This process was inhibited within minutes by tetanus toxin and its isolated light chain, but not by its heavy chain. The data demonstrate that the effect of tetanus toxin on NA release from purified synaptosomes is caused by the intraterminal action of its light chain.

[学科分类] 生物化学/生物物理

[关键词] Tetanus toxin;Streptolysin O;Permeabilization;Exocytosis;Synaptosome;BoNT/A;botulinum A toxin;H;heavy chain;L;light chain;NA;noradrenaline;SLO;streptolysin O;TeTx;tetanus toxin