Stimulation of protein kinase C (PKC) activity in resting Swiss 3T3 cells by prostaglandin F2α

[摘要]

Prostaglandin F_2α (PGF_2α), a mitogen for resting Swiss 3T3 cells, rapidly stimulates phosphorylation of an 80 kDa protein (80 K). 1-Oleoyl-2-acetylglycerol (OAG) and 12-O-tetradecanoyl phorbol-13-acetate (TPA) both protein kinase C (PKC) activators, also elicit 80 K phosphorylation. In contrast PGE₁, PGE₂ or PGF_2β, which are non-mitogenic in these cells, had tittle or no action on this event. However PGE₁ and PGE₂ potentiate the PGF_2α proliferative effect but do not enhance its action on 80 K phosphorylation. These results suggest that PGF_2α mitogenic induction involves PKC signalling pathway activation while its enhancement by PGE₁ or PGE₂ occurs through a different mechanism(s).

[学科分类] 生物化学/生物物理

[关键词] Swiss 3T3 cell;Prostaglandin F2α;Protein kinase C;80 K phosphorylation;DNA synthesis;PGF2α;prostaglandin F2α;PGF2β;prostaglandin F2β;PGE1;prostaglandin E1;PGE2;prostaglandin E2;PKC;protein kinase C;OAG;1-oleoyl-2-acetylglycerol;DAG;diacylglycerol;TPA;12-O-tetradecanoylphorbol-13-acetate;PB12;phorbol-12;13-dibutyrate