The effect of the UL42 protein on the DNA polymerase activity of the catalytic subunit of the DNA polymerase encoded by herpes simplex virus type 1

[摘要]

The effect that the UL42 protein of herpes simplex virus type 1 has on the DNA polymerase activity of the DNA polymerase catalytic subunit (Pol) of the same virus has been investigated. The observed effects are critically dependent on the salt used and its concentration, such that the UL42 protein may inhibit, have little or no effect on, or activate the Pol activity, depending on the conditions used. The observed effects are due to the values for K m_app for activated DNA and V max_app for Pol and the Pol-UL42 protein complex differently varying with salt concentration.

[学科分类] 生物化学/生物物理

[关键词] DNA polymerase;UL42 protein;Herpes simplex virus;HSV-1;Herpes simplex virus type 1;Pol;catalytic subunit of DNA polymerase of HSV-1;PMSF;phenylmethylsulfonyl fluoride;HEPES;N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid