Latrotoxin‐like properties of a protein from brain

[摘要]

In bovine brain cortex cytoplasm we have identified a soluble protein (L-protein) of M _r ∼90 kDa interacting with polyclonal antibodies to α-latrotoxin. The L-protein forms potential-dependent and cation-selective ion channels in BLM, which are blocked by Cd²⁺. The fusogenic activity of the L-protein was demonstrated on liposomes. We have arrived at the conclusion that the action mechanisms of the L-protein and α-latrotoxin are similar.

[学科分类] 生物化学/生物物理

[关键词] Latrotoxin;L-protein;Bilayer lipid membrane;Liposome fusion;Channel;Bovine brain;BLM;bilayer lipid membrane;Tris;tris(hydroxymethyl)aminomethine;PC;phosphatidylcholine;PE;phosphatidylcthanolamine;SDS;sodium dodecyl sulfate;DPA;dipicolinie acid