Determination of the DNA binding site of the GAL4 protein A photo‐CIDNP study

[摘要]

After the assignment of ¹H NMR signals of aromatic side chains by means of specific deuteration, we analyzed the DNA binding site of GAL4 by measuring photo-CIDNP spectra. The results showed that Trp³⁶ is involved in both the specific interaction with UAS_G and non-specific DNA binding. This residue is located inside the Cys-rich region, but outside the putative Zn-finger. The photo-CIDNP spectrum also showed that the side chains of Tyr⁴⁰ and His⁵³ are not exposed on the surface of the protein.

[学科分类] 生物化学/生物物理

[关键词] NMR;Photo-CIDNP;GAL4;UASG;Zinc finger;DNA binding