[摘要] Carbohydrates were characterized in the human placental α2-macroglobulin receptor and its associated protein. Carbohydrates, largely N-linked, contributed to about 18% of the size of the receptor α-chain and to about 25% of the β-chain. The 40 kDa receptor-associated Protein also contained carbohydrate. The α- and β-chains contained a wide variety of carbohydrates as judged by binding of lectins. Monosaceharide-competing inhibition of α2M-methylamine binding by WGA suggested a functional significance of sugars in binding of ligands to the α-chain.
[发布日期] [发布机构]
[效力级别] [学科分类] 生物化学/生物物理
[关键词] α2-Macroglobulin receptor;Low density lipoprotein receptor-related protein;Glycosylation;Lectin;α2M;α2-macroglobulin;α2MR;α2-macroglobulin receptor;α2MRAP;α2-macroglobulin receptor-associated protein;LRP;low density lipoprotein receptor-related protein;SDS;sodium dodecyl sulfate;PAGE;polyacrylamide gel electrophoresis;O-glycanase;endo-α-N-acetylgalactosaminidase;Endo-H;endo-β-N-acetylglucosaminidase H;PNGase-F;peptide N-glycohydrolase F;Lectins (agglutinins);ACA;amarantus caudatus;PNA;arachis hypogeae;BS-I;bandeiraea simplicifolia I;ConA;canavalia ensiformis;DSA;datura stramonium;ECG;erythrina crista galli;GNA;galanthus nivalis;LCA;lens culinaris;MAA;maackia amurensis;SNA;sambucus nigra;WGA;triticum vulgaris;PWM;phytolacca americana;VVA;vicia villosa [时效性]
