Human urokinase contains Ga1NAcβ(1‐4)[Fucα(1‐3)]G1cNAcβ(1‐2) as a novel terminal element in N‐linked carbohydrate chains

[摘要]

Structural analysis of enzymically released N-linked carbohydrate chains of human urokinase (urinary-type plasminogen activator) by ¹H NMR spectroscopy and FAB-MS demonstrated that the N-linked oligosaccharides on the only N-glycosylation site contain diantennary structures with the novel Ga1NAcβ(1-4)[Fucα(1-3)]G1cNAcβ(1-2) element in the upper or the lower branch.

[学科分类] 生物化学/生物物理

[关键词] Urokinase;Plasminogen activator;N-Linked carbohydrate chain;Fast atom bombardment mass spectroscopy (FAD-MS);1H NMR spectroscopy;u-PA;urinary type plasminogen activator;t-PA;tissue-type plasminogen activator;EGF;epidermal growth factor;Fuc;fucose;Neu5Ac;N-acetylneuraminic acid;CHO;Chinese hamster ovary;PNGase-F;peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase F;n.O.e;nuclear Overhauser enhancement;HOHAHA;homonuclear Hartmann-Hahn;MLEV;composite pulse devised by Malcom Levitt;NOESY;nuclear Overhauser enhancement spectroscopy;FAB-MS;fast atom bombardment mass spectrometry;GLC;gas liquid chromatography;FPLC;fast protein liquid chromatography;HPLC;high-pressure liquid chromatography;Le x;Lewis x