Low translational efficiency of the F1‐ATPase β‐subunit mRNA largely accounts for the decreased ATPase content in brown adipose tissue mitochondria

[摘要]

The half-life of the F₁-ATPase β-subunit (F₁-β) mRNA in ATPase-poor brown adipose tissue (BAT) (t?? = 9.5 h) was found to be 3–7-fold shorter than in liver (t?? = 27 h) and heart (t?? = 63 h) of mice. When translated in reticulocyte lysate, a 2–3-fold lower efficiency appeared with F₁-β mRNA from BAT than from other tissues. The in vitro synthesized F₁-β protein precursors of BAT, liver and heart origin were imported and processed by mouse liver mitochondria with equal efficiency. The results indicate that the pool or abundant F₁-β mRNA in BAT is not fully translatable most likely due to its low metabolic stability.

[学科分类] 生物化学/生物物理

[关键词] Brown adipose tissue;ATPase;mRNA half-life;Translational efficiency;BAT;brown adipose tissue;ATPase;mitochondrial ATP synthase;F1-β;β subunit of the catalytic F1-part of mitochondrial ATP synthase;UCP;uncoupling protein