Involvement of MAP kinase activators in angiotensin II‐induced activation of MAP kinases in cultured vascular smooth muscle cells

[摘要]

In cultured vascular smooth muscle cells (VSMC) angiotensin II (ang II) induces tyrosine and serine/threonine phosphorylation and activation of two mitogen-activated protein (MAP) kinases, When extracts of ang II-stimulated VSMC were fractionated by Mono Q anion-exchange column chromatography, three peaks of the activities which in vitro activate inactive MAP kinases were detected. These MAP kinase activator activities were not detected in extracts of unstimulated VSMC. In vitro activation of MAP kinases by the MAP kinase activators was accompanied by tyrosine and serine/threonine phosphorylation of MAP kinases. These results suggest that the MAP kinase activators are involved in the ang II-induced phosphorylation and activation of MAP kinases in VSMC.

[学科分类] 生物化学/生物物理

[关键词] Angiotensin II;Mitogen-activated protein kinase;MAP kinase activator;Protein kinase C;Vascular smooth muscle cell;VSMC;vascular smooth muscle cells;ang II;angiotensin II;MAP kinase;mitogen-activated protein kinase;EGF;epidermal growth factor;NCF;nerve growth factor;PMA;phorbol 12-myristate 13-acetate;MBP;myelin basic protein;EGTA;[ethylene bis(oxyethylenenitrilo)]-tetraacetic acid;DTT;dithiothreitol;APMSF;(p-amidinophenyl)-mythenesulfonyl fluoride;SDS;sodium dodecylsulfate;PAGE;polyacrylamide gel electrophoresis;PVDF;polyvinylidene dioxide