The inverted hexagonal phase is more sensitive to hydroperoxidation than the multilamellar phase in phosphatidylcholine and phosphatidylethanolamine aqueous dispersions

[摘要]

The effect of phase behaviour (hexagonal II phase and lamellar phase) on the peroxidation of membrane phospholipids has been investigated in dilinoleoyl phosphatidylcholine (DLPC)/dilinoleoyl phosphatidylethanolamine (DLPE) aqueous dispersions. Peroxidation was initiated with a water-soluble radical inducer 2,2′-azobis (2-amidino-propane) dihydrochloride (AAPN). The phospholipid morphology was monitored by ³¹P-nuclear magnetic resonance (NMR). Phospholipid hydroperoxides (PCOOH and PEOOH) were determined by chemiluminescence high-performance liquid chromatography (CL—HPLC). In pH-induced phase transition systems, DLPE in the bilayer state was much less oxidized than in the hexagonal II state. In composition-induced phase transition systems, the formation of total hydroperoxides and the consumption of α-tocopherol in the hexagonal II phase were greater than in the bilayer phase. These data suggest that the hexagonal II phase is more sensitive to hydroperoxidation than the bilayer phase in phospholipid aqueous dispersions.

[学科分类] 生物化学/生物物理

[关键词] Lamellar;Hexagonal;Hydroperoxide;Phosphatidylcholine;Phosphatidylethanolamine;Peroxidation;Lα;lamella;HII;inverted hexagonal;PC;phosphatidylcholine;PE;phosphatidylethanolamine;AAPN;2;2′-azobis (2-amidino-propane) dihydrochloride;NMR;nuclear magnetic resonance;DLPC;l-α-dilinoleoyl phosphatidylcholine;DLPE;l-α-dilinoleoyl phosphatidylethanolamine;α-Toc;d-α-tocopherol;PMC;2;2;5;7;8-pentamethyl-6-hydroxychroman;BHT;butylated hydroxytoluene;MLV;multilamellar vesicles;CL—HPLC;chemiluminescence high-performance liquid chromatography;PCOOH;phosphatidylcholine hydroperoxide;PEOOH;phosphatidylethanolamine hydroperoxide