Inhibition of hormone‐sensitive lipase by intermediary lipid metabolites

[摘要]

Hormone-sensitive lipase (HSL) is inhibited in a non-competitive manner by oleoyl CoA, oleic acid and 2-monopalmitoylglycerol, 50% inhibition being observed at concentrations of approx. 0.1 μM, 0.5 μM and 500 μM, respectively. HSL is a key enzyme in lipid metabolism, mobilising triacylglycerol and cholesterol ester stores in several tissues. Feedback inhibition of HSL by oleoyl CoA and oleic acid may therefore prevent accumulation of free fatty acids and cholesterol in the cell, whereas 2-monoacylglycerol may act as a feedback inhibitor if the capacity of monoacylglycerol lipase is exceeded.

[学科分类] 生物化学/生物物理

[关键词] Hormone-sensitive lipase;Oleic acid;Oleoyl CoA;2-Monopalmitoylglycerol;Feedback inhibition;HSL;hormone-sensitive lipase;CoA;coenzyme A;HMG-CoA;hydroxymethylglutaryl-CoA;PNPB;p-nitrophenylbutyrate;CMC;critical micellar concentration;PDH;pyruvate dehydrogenase