Expression of functional Thermoplasma acidophilum proteasomes in Escherichia coli

[摘要]

The two genes encoding the constituent subunits of the Thermoplasma acidophilum proteasome were expressed in Escherichia coli yielding fully assembled molecules as shown by electron microscopy. The recombinant proteasomes were purified to homogeneity and ware shown to have proteolytic activity indistinguishable from proteasomes isolated from T. acidophilum.

[学科分类] 生物化学/生物物理

[关键词] Proteasome;Gene expression;Archaebacterium;Thermoplasma acidophilum;FPLC;fast protein liquid chromatography;NMec;4-methyl-7-coumarylamide;OD600;optical density measured at 600 nm;pI;isoelectric point;RBS;ribosome binding site