De novo synthesis and desaturation of fatty acids at the mitochondrial acyl‐carrier protein, a subunit of NADH:ubiquinone oxidoreductase in Neurospora crassa

[摘要]

We have cultivated the cel mutant of Neurospora crassa defective in cytosolic fatty acid synthesis with [2-¹⁴C]malonate and found radioactivity covalently attached to the mitochondrial acyl-carrier protein (ACP), a subunit of the respiratory chain NADH:ubiquinone oxidoreductase. We purified the ACP by reverse-phase HPLC: the bound acyl groups were trans-esterified to methylesters and analyzed by gas chromatography. The saturated C₀ to C₁₈ fatty acids and oleic acid were detected. De novo synthesis and desaturation of fatty acids at the ACP subunit of NADH:ubiquinone oxidoreductase and use of the products of this mitnchondrial synthetic pathway for cardiolipin synthesis is discussed.

[学科分类] 生物化学/生物物理

[关键词] NADH:ubiquinone oxidoreductase;Complex I;Mitochondrial fatty acid synthesis;Cardiolipin